CASP9 Knockout cell line (Hep G2)

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CASP9 Knockout cell line (Hep G2) Order

CASP9 Knockout cell line (Hep G2)
Catalog#
YKO-HT1458
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CASP9 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CASP9 Knockout cell line (Hep G2)
Species
Human
Gene Name
CASP9
Gene ID
842
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CASP9-201)
Validation: PCR+Sanger Sequencing
CASP9 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CASP9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CASP9 gene knockout CASP9 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CASP9 Knockout cell line (Hep G2)
CASP9 Knockout cell line (Hep G2)
Catalog#
YKO-HT1458
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • CASP9 Knockout cell line (THP-1)

    CASP9 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HT0203
    YKO-HT0203(THP-1)
  • Casp9 Knockout cell line (Raw 264.7)

    Casp9 Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Casp9
    Gene ID: 12371
    Size: 1*10^6
    Catalog#: YKO-MT0025
    YKO-MT0025(Raw 264.7)
  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
    YKO-H705(A549)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • CASP9 Knockout cell line (THP-1)

    CASP9 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HT0203
      inquiry   More details
  • Casp9 Knockout cell line (Raw 264.7)

    Casp9 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Casp9
    Gene ID: 12371
    Size: 1*10^6
    Catalog#: YKO-MT0025
      inquiry   More details
  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
      inquiry   More details

CASP9 Knockout cell line (Hep G2) Order

CASP9 Knockout cell line (Hep G2)
Catalog#
YKO-HT1458
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CASP9 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CASP9 Knockout cell line (Hep G2)
Species
Human
Gene Name
CASP9
Gene ID
842
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CASP9-201)
Validation: PCR+Sanger Sequencing
CASP9 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CASP9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CASP9 gene knockout CASP9 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CASP9 Knockout cell line (Hep G2)
CASP9 Knockout cell line (Hep G2)
Catalog#
YKO-HT1458
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • CASP9 Knockout cell line (THP-1)

    CASP9 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HT0203
    YKO-HT0203(THP-1)
  • Casp9 Knockout cell line (Raw 264.7)

    Casp9 Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Casp9
    Gene ID: 12371
    Size: 1*10^6
    Catalog#: YKO-MT0025
    YKO-MT0025(Raw 264.7)
  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
    YKO-H705(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • CASP9 Knockout cell line (THP-1)

    CASP9 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HT0203
      inquiry   More details
  • Casp9 Knockout cell line (Raw 264.7)

    Casp9 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Casp9
    Gene ID: 12371
    Size: 1*10^6
    Catalog#: YKO-MT0025
      inquiry   More details
  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
      inquiry   More details

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